53 - Freie Mitteilung
Inflammation & transplantation
16. Mai 2019, 10:15 - 11:45, Szenario 1, 5. OG
Portosystemic shunts effects on on a high fat diet mouse model: Preliminary data
A. Peloso, C. Toso, Presenter: A. Peloso (Genéve)
It's stated that we need to face a worldwide epidemic of obesity with an increased risk of comorbidities related to metabolic syndromes. In this setup, the liver is known as a critical organ where a toxic buildup of fat into hepatocytes progresses from a simple steatosis (fatty liver disease), passing trough non-alcoholic steatohepatitis (NASH) to final cirrhosis. All these conditions, in the absence of an excessive alcohol consumption fall under the umbrella of non-alcoholic fatty liver disease (NAFLD). Portal hypertension is a specific mark for NAFLD with systemic effects on gut, tumor development and fibrosis. Our goal is to determine the impact of porto-systemic vein shunt on portal hypertension, NAFLD and cardiovascular outcomes in adult mice fed with high-fat diet (HFD).
C57BL/6 mice received a high fat or a standard diet starting from 4 weeks of age, and shunts (or sham surgery) were created at 8 weeks. A lombotomy was created, and the spleen placed in the subcutaneous tissue. The development of collateral veins was stimulated by scarring the spleen’s surface on both sides. The shunt presence and permeability was checked by Micro-CT scan and by fluorescent micro-bead injection respectively after 40 weeks. Liver volume, weight, portal pressure and histology were assessed. Vascular stiffness was also measured by myograph study of thoracic aorta.
75% of mice developed evident porto-systemic shunt at mid-term (17 weeks). Our preliminary data showed a decrease of hepatic volumes in HFD diet mice with porto-systemic shunt (P<0.05) with a decrease of portal pressure. Vascular rigidity seems to be increased in porto-systemic shunt populations.
Even with preliminary data, the proposed model offers liver and cardiovascular outcomes.