49 - Freie Mitteilung
Clinical works II
16. Mai 2019, 10:15 - 11:45, Bellavista 2, 6. OG
Characterization of laser perforated devitalized tracheal cartilage as a matrix for tissue-engineered trachea substitute.
D. Baranovskii1, J. Demner1, S. Nürnberger2, A. Lyindup3, M. Baumgartl1, H. Redl2, A. Barbero1, I. Martin1, D. Lardinois1, Presenter: D. Baranovskii1 (1Basel, 2Vienna/AT, 3Moscow/RU)
Tissue-engineered trachea transplantation represents a visionary strategy for the treatment of patients with tracheal wall defect after resection. A tracheal substitute should be stiff vital cartilage tissue. For the production of such graft, it would be desirable to isolate chondrogenic cells from an easily accessible tissue biopsy. We demonstrated that adult human nasal chondrocytes (hNC) display a unique regenerative properties. However, they are not capable to colonize native stiff devitalized tracheal cartilage. The aim of this study was investigate the capability of laser-perforated devitalized tracheal cartilage tissues (LPTCT) to form a niche for cell colonization.
Human native tracheal specimens, isolated from cadaveric donors, were exposed to devitalization, controlled superficial or deep laser-perforation (300 - 1000 μm depth) and irradiation with Iridium-192 for disinfection. hNC have been harvested from septal cartilage biopsies of patients undergoing rhinoplasty. Following seeding and culture with hNC, devitalized tracheal cartilage tissues were analyzed histologically or implanted ectopically in nude mice. Additionally, deep perforated cartilage specimens were implanted in orthotopic position in tracheal wall defects in rabbits.
Laser-perforated tracheal cartilage tissue was efficiently colonized by NC after 7 days of culture. Extent of colonization (i.e.: percentage of viable cells spanning >300 microns of tissue depth) further improved once the revitalized constructs were implanted ectopically in nude mice. Being implanted in orthotopic position, devitalised deep perforated matrices demonstrated sufficiently stable position in a tracheal wall without any evidence of dislocation or relevant tracheal stenosis even 8 week after implantation according to Micro-CT analysis.
We demonstrated that the pores of the LPTCT could be efficiently colonised by hNC after 7 days of culture in vitro. Extent of colonization further improved once the revitalized constructs were implanted in vivo. New experiments are ongoing to establish proper conditions allowing efficient epithelialization of the revitalited LPTCT using nasal epithelial cells (i.e., cell source that can be isolated together with hNC from the same biopsy) and to assess the capacity of the resulting bi-phasic constructs to functional repair tracheal wall defects in rabbits.